Mycobacterium tuberculosis Rv2145c Promotes Intracellular Survival by STAT3 and IL-10 Receptor Signaling
Though Mycobacterium tuberculosis (Mtb) is an intracellular pathogen in phagocytic cells, the elements and mechanisms by which they invade and persist in host cells are nonetheless not effectively understood. Characterization of the bacterial proteins modulating macrophage operate is important for understanding tuberculosis pathogenesis and bacterial virulence. Right here we investigated the pathogenic position of the Rv2145c protein in stimulating IL-10 manufacturing.
We first discovered that recombinant Rv2145c stimulated bone marrow-derived macrophages (BMDMs) to secrete IL-10, IL-6 and TNF-α however not IL-12p70 and to extend the expression of floor molecules by way of the MAPK, NF-κB, and TLR4 pathways and enhanced STAT3 activation and the expression of IL-10 receptor in Mtb-infected BMDMs. Rv2145c considerably enhanced intracellular Mtb development in BMDMs in contrast with that in untreated cells, which was abrogated by STAT3 inhibition and IL-10 receptor (IL-10R) blockade.
Expression of Rv2145c in Mycobacterium smegmatis (M. smegmatis) led to STAT3-dependent IL-10 manufacturing and enhancement of intracellular development in BMDMs. Moreover, the clearance of Rv2145c-expressing M. smegmatis within the lungs and spleens of mice was delayed, and these results had been abrogated by administration of anti-IL-10R antibodies. Lastly, all mice contaminated with Rv2145c-expressing M. smegmatis died, however these contaminated with the vector management pressure didn’t. Our knowledge recommend that Rv2145c performs a job in creating a positive atmosphere for bacterial survival by modulating host alerts.
Unraveling the position of 12- and 20-HETE in cardiac pathophysiology: G-protein coupled receptors, pharmacological inhibitors and transgenic approaches
Arachidonic acid-derived lipid mediators play essential roles within the growth and development of cardiovascular illnesses. Eicosanoid metabolites generated by lipoxygenases and cytochrome P450 enzymes produce a number of lessons of molecules, together with the epoxyeicosatrienoic acid (EET) and hydroxyeicosatetraenoic acids (HETE) household of bioactive lipids. Normally, the cardioprotective results of EETs have been documented throughout plenty of cardiac illnesses.
- In distinction, members of the HETE household have been proven to contribute to the pathogenesis of ischemic cardiac illness, maladaptive cardiac hypertrophy and coronary heart failure.
- The web impact of 12(S)- and 20-HETE relies upon upon the relative quantities generated, ratio of HETEs/EETs produced, timing of synthesis, in addition to mobile and subcellular mechanisms activated by every respective metabolite.
- HETEs are synthesized by and have an effect on a number of cell varieties inside the myocardium. Furthermore, cytochrome P450- (CYP) and lipoxygenase- (LOX) derived metabolites have been proven to straight affect cardiac myocyte development and the regulation of cardiac fibroblasts.
- The mechanistic knowledge uncovered to date has employed using enzyme inhibitors, HETE antagonists and the genetic manipulation of lipid-producing enzymes and their respective receptors, all of which affect a fancy community of outcomes that complicate knowledge interpretation. This assessment will summarize and combine latest findings on the position of 12(S)-/20-HETE in cardiac illnesses.
 Tryptone, Casein Digest Peptone, Tryptic digest of Casein |
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| T2007-100 | GenDepot | 1Kg | EUR 300 |
 CASEIN DIGEST PEPTONE |
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| C03-104-10kg | Alphabiosciences | 10 kg | EUR 1664 |
CASEIN DIGEST PEPTONE |
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| C03-104-2kg | Alphabiosciences | 2kg | EUR 401 |
CASEIN DIGEST PEPTONE |
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| C03-104-500g | Alphabiosciences | 500 g | EUR 145 |
 CASEIN DIGEST PEPTONE 1 GU |
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| C03-145-10kg | Alphabiosciences | 10 kg | EUR 1315 |
CASEIN DIGEST PEPTONE 1 GU |
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| C03-145-2kg | Alphabiosciences | 2kg | EUR 325 |
CASEIN DIGEST PEPTONE 1 GU |
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| C03-145-500g | Alphabiosciences | 500 g | EUR 125 |
) Tryptone (Casein hydrolysate, enzymatic digest) |
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| GE6771-100G | Glentham Life Sciences | 100 g | EUR 54 |
Tryptone (Casein hydrolysate, enzymatic digest) |
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| GE6771-250G | Glentham Life Sciences | 250 g | EUR 75 |
Tryptone (Casein hydrolysate, enzymatic digest) |
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| GE6771-500G | Glentham Life Sciences | 500 g | EUR 110 |
 PANCREATIC DIGEST OF GELATIN |
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| P16-100-10kg | Alphabiosciences | 10 kg | EUR 689 |
PANCREATIC DIGEST OF GELATIN |
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| P16-100-2kg | Alphabiosciences | 2kg | EUR 189 |
PANCREATIC DIGEST OF GELATIN |
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| P16-100-500g | Alphabiosciences | 500 g | EUR 88 |
 PAPAIC DIGEST OF SOYBEANS |
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| P16-101-10kg | Alphabiosciences | 10 kg | EUR 804 |
PAPAIC DIGEST OF SOYBEANS |
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| P16-101-2Kg | Alphabiosciences | 2 Kg | EUR 214 |
PAPAIC DIGEST OF SOYBEANS |
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| P16-101-500g | Alphabiosciences | 500 g | EUR 95 |
 λ-DNA/ EcoR I Digest |
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| 300011 | GeneOn | 100µg | EUR 56 |
λ-DNA/ EcoR I Digest |
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| 300012 | GeneOn | 5x100µg | EUR 154 |
 λ-DNA/ Sty I Digest |
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| 300015 | GeneOn | 200µg | EUR 56 |
λ-DNA/ Sty I Digest |
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| 300016 | GeneOn | 5x200µg | EUR 154 |
 λ-DNA/ Pst I Digest |
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| 300017 | GeneOn | 200µg | EUR 56 |
λ-DNA/ Pst I Digest |
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| 300018 | GeneOn | 5x200µg | EUR 154 |
 λ-DNA/ BstE II Digest |
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| 300019 | GeneOn | 200µg | EUR 56 |
λ-DNA/ BstE II Digest |
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| 300020 | GeneOn | 5x200µg | EUR 154 |
 λ-DNA/ Hind III Digest |
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| 300030 | GeneOn | 200µg | EUR 56 |
λ-DNA/ Hind III Digest |
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| 300031 | GeneOn | 5x200µg | EUR 154 |
 PEPTIC DIGEST OF ANIMAL TISSUE |
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| P16-102-10kg | Alphabiosciences | 10 kg | EUR 788 |
PEPTIC DIGEST OF ANIMAL TISSUE |
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| P16-102-2kg | Alphabiosciences | 2kg | EUR 210 |
PEPTIC DIGEST OF ANIMAL TISSUE |
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| P16-102-500g | Alphabiosciences | 500 g | EUR 94 |
 Casein |
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| 20-abx186406 | Abbexa |
|
|
|
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Casein |
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| GE4125-100G | Glentham Life Sciences | 100 g | EUR 42 |
Casein |
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| GE4125-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Casein |
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| GE4125-250G | Glentham Life Sciences | 250 g | EUR 50 |
Casein |
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| GE4125-500G | Glentham Life Sciences | 500 g | EUR 62 |
Casein |
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| GE4125-5KG | Glentham Life Sciences | 5 kg | EUR 241 |
Casein |
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| CN5204 | Bio Basic | 500g | EUR 93.5 |
|
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 λ-DNA/ EcoR I/ Hind III Digest |
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| 300013 | GeneOn | 200µg | EUR 56 |
λ-DNA/ EcoR I/ Hind III Digest |
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| 300014 | GeneOn | 5x200µg | EUR 154 |
 Mounting Medium |
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| 4300 | Virostat | 3 ml | EUR 180.5 |
|
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
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 VALI Medium |
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| 450 | AthenaES | 500 ml | EUR 265 |
 BEGM Medium |
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| 451 | AthenaES | 500 ml | EUR 152 |
 DC MEDIUM |
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| D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
DC MEDIUM |
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| D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
DC MEDIUM |
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| D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
 EC MEDIUM |
|||
| E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
EC MEDIUM |
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| E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
EC MEDIUM |
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| E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
 Advanced Medium |
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| C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
 Medium 199 |
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| C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
 COLIFORM MEDIUM |
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| C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
COLIFORM MEDIUM |
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| C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
COLIFORM MEDIUM |
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| C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
 Heller's Medium |
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| CP014-010 | GenDepot | 10X1L | EUR 99 |
Heller's Medium |
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| CP014-500 | GenDepot | 50L | EUR 126 |
 Hoagland's Medium |
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| CP015-010 | GenDepot | 10X1L | EUR 99 |
Hoagland's Medium |
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| CP015-500 | GenDepot | 50L | EUR 126 |
 HLP MEDIUM |
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| H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
HLP MEDIUM |
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| H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
HLP MEDIUM |
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| H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
 NeuroProgenitor Medium |
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| NM42400 | Neuromics | 125 ml | EUR 304 |
 SIM MEDIUM |
|||
| S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
SIM MEDIUM |
|||
| S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
SIM MEDIUM |
|||
| S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
 SOB MEDIUM |
|||
| S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
SOB MEDIUM |
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| S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
SOB MEDIUM |
|||
| S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
 A Medium |
|||
| DJ1018 | Bio Basic | 100g | EUR 84.8 |
|
|||
 M9 Medium |
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| SD7024 | Bio Basic | 250g | EUR 71.75 |
|
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 M9CA Medium |
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| SD7025 | Bio Basic | 250g | EUR 71.75 |
|
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 YM Medium |
|||
| SD7031 | Bio Basic | 250g | EUR 70.45 |
|
|||
 TYGPN Medium |
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| SD7032 | Bio Basic | 250g | EUR 70.01 |
|
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 M63 Medium |
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| SD7033 | Bio Basic | 250g | EUR 71.75 |
|
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 Casein protein |
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| 30R-3113 | Fitzgerald | 1 mg | EUR 651 |
|
Description: Purified native Casein protein |
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 Casein antibody |
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| 10-2483 | Fitzgerald | 100 ug | EUR 241 |
|
Description: Mouse monoclonal Casein antibody |
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 Casein Antibody |
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| 20-abx110391 | Abbexa |
|
|
|
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Casein Antibody |
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| 20-abx134730 | Abbexa |
|
|
|
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Casein Antibody |
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| 1-CSB-PA00880E0Rb | Cusabio |
|
|
|
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|
Description: A polyclonal antibody against Casein. Recognizes Casein from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
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 Insect Cell Medium: TNM-FH Insect Culture Medium |
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| ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
|
|||
 Insect Cell Medium: Serum-Free Insect Culture Medium |
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| ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
|
|||
) Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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| ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
|
|||
 EverBrite Mounting Medium |
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| 23001 | Biotium | 10mL | EUR 178 |
|
Description: Minimum order quantity: 1 unit of 10mL |
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 Recovery Medium - 6x12mL |
|||
| 1711 | Intact Genomics | 4/PK | EUR 128 |
 SuperMOUNT? Mounting Medium |
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| 1211-20 | Biovision | EUR 201 | |
 Aqueous Mounting Medium |
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| AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 80 |
 Antifade Mounting Medium |
|||
| AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 65 |
 EC MEDIUM, MODIFIED |
|||
| E05-108-10kg | Alphabiosciences | 10 kg | EUR 858 |
EC MEDIUM, MODIFIED |
|||
| E05-108-2Kg | Alphabiosciences | 2 Kg | EUR 226 |
EC MEDIUM, MODIFIED |
|||
| E05-108-500g | Alphabiosciences | 500 g | EUR 98 |
 A-1 MEDIUM |
|||
| A01-116-10kg | Alphabiosciences | 10 kg | EUR 1034 |
A-1 MEDIUM |
|||
| A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 264 |
A-1 MEDIUM |
|||
| A01-116-500g | Alphabiosciences | 500 g | EUR 108 |
 RPMI 1640 Medium |
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| abx098879-500ml | Abbexa | 500 ml | EUR 175 |
|
|||
 MCDB 131 Medium |
|||
| CM034-050 | GenDepot | 500ml | EUR 85 |
MCDB 131 Medium |
|||
| CM034-300 | GenDepot | 6x500ml | EUR 165 |
MCDB 131 Medium |
|||
| CM034-310 | GenDepot | 10x500ml | EUR 219 |
MCDB 131 Medium |
|||
| CM034-320 | GenDepot | 20x500ml | EUR 340 |
MCDB 131 Medium |
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| CM034-350 | GenDepot | 50x500ml | EUR 585 |
 Williams' Medium E |
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| CM063-050 | GenDepot | 500ml | EUR 91 |
Williams' Medium E |
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| CM063-300 | GenDepot | 6x500ml | EUR 266 |
Williams' Medium E |
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| CM063-310 | GenDepot | 10x500ml | EUR 313 |
Williams' Medium E |
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| CM063-320 | GenDepot | 20x500ml | EUR 604 |
Williams' Medium E |
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| CM063-350 | GenDepot | 50x500ml | EUR 975 |
 AddexBio Cryopreservation Medium |
|||
| C0002-20mL | Addexbio | RT vial | EUR 104 |
 CARY & BLAIR MEDIUM |
|||
| C03-131-10kg | Alphabiosciences | 10 kg | EUR 2185 |
CARY & BLAIR MEDIUM |
|||
| C03-131-2Kg | Alphabiosciences | 2 Kg | EUR 514 |
CARY & BLAIR MEDIUM |
|||
| C03-131-500g | Alphabiosciences | 500 g | EUR 176 |
 Cereal Crops Medium |
|||
| CP003-005 | GenDepot | 5L | EUR 113 |
Cereal Crops Medium |
|||
| CP003-010 | GenDepot | 10X1L | EUR 169 |
 Kao Michayluk Medium |
|||
| CP017-010 | GenDepot | 10X1L | EUR 99 |
Kao Michayluk Medium |
|||
| CP017-500 | GenDepot | 50L | EUR 126 |
 Orchid Maintenance Medium |
|||
| CP037-010 | GenDepot | 10X1L | EUR 109 |
Orchid Maintenance Medium |
|||
| CP037-500 | GenDepot | 50L | EUR 138 |
 Woody Plants Medium |
|||
| CP040-010 | GenDepot | 10X1L | EUR 101 |
Woody Plants Medium |
|||
| CP040-500 | GenDepot | 50L | EUR 126 |
 Hepatocyte Maintenance Medium |
|||
| HM42600 | Neuromics | 100 ml | EUR 409 |
 Neuron Growth Medium |
|||
| HNM001 | Neuromics | 500 ml | EUR 331 |
 FLUID THIOGLYCOLLATE MEDIUM |
|||
| F06-101-10kg | Alphabiosciences | 10 kg | EUR 792 |
FLUID THIOGLYCOLLATE MEDIUM |
|||
| F06-101-2kg | Alphabiosciences | 2kg | EUR 211 |
FLUID THIOGLYCOLLATE MEDIUM |
|||
| F06-101-500g | Alphabiosciences | 500 g | EUR 94 |
 Agarose, medium EEO |
|||
| GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
Agarose, medium EEO |
|||
| GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
 FluoreGuard Mounting Medium |
|||
| FMM030 | ScyTek Laboratories | 30 ml | EUR 96 |
FluoreGuard Mounting Medium |
|||
| FMM060 | ScyTek Laboratories | 60 ml | EUR 116 |
FluoreGuard Mounting Medium |
|||
| FMM500 | ScyTek Laboratories | 500 ml | EUR 327 |
FluoreGuard Mounting Medium |
|||
| FMM999 | ScyTek Laboratories | 1000 ml | EUR 530 |
 OF BASAL MEDIUM |
|||
| O15-100-10kg | Alphabiosciences | 10 kg | Ask for price |
OF BASAL MEDIUM |
|||
| O15-100-2kg | Alphabiosciences | 2kg | Ask for price |
OF BASAL MEDIUM |
|||
| O15-100-500g | Alphabiosciences | 500 g | Ask for price |
 MOTILITY TEST MEDIUM |
|||
| M13-121-10kg | Alphabiosciences | 10 kg | EUR 1528 |
MOTILITY TEST MEDIUM |
|||
| M13-121-2Kg | Alphabiosciences | 2 Kg | EUR 371 |
MOTILITY TEST MEDIUM |
|||
| M13-121-500g | Alphabiosciences | 500 g | EUR 137 |
 REINFORCED CLOSTRIDIUM MEDIUM |
|||
| R18-105-10kg | Alphabiosciences | 10 kg | EUR 1194 |
REINFORCED CLOSTRIDIUM MEDIUM |
|||
| R18-105-2kg | Alphabiosciences | 2kg | EUR 299 |
REINFORCED CLOSTRIDIUM MEDIUM |
|||
| R18-105-500g | Alphabiosciences | 500 g | EUR 118 |
 Sepadextran-25 Medium |
|||
| SD25M-100 | ABTBeads | 100 g | EUR 271 |
Sepadextran-25 Medium |
|||
| SD25M-500 | ABTBeads | 500 g | EUR 1036 |
 Sepadextran-50 Medium |
|||
| SD50M-100 | ABTBeads | 100 g | EUR 344 |
Sepadextran-50 Medium |
|||
| SD50M-500 | ABTBeads | 500 g | EUR 1285 |
 Schwann Growth Medium |
|||
| SGM001 | Neuromics | 500 ml | EUR 244 |
 TERRIFIC BROTH MEDIUM |
|||
| T20-114-10kg | Alphabiosciences | 10 kg | EUR 675 |
TERRIFIC BROTH MEDIUM |
|||
| T20-114-2kg | Alphabiosciences | 2kg | EUR 186 |
TERRIFIC BROTH MEDIUM |
|||
| T20-114-500g | Alphabiosciences | 500 g | EUR 87 |
 THIOGLYCOLLATE MEDIUM, BREWER |
|||
| T20-118-10kg | Alphabiosciences | 10 kg | EUR 1364 |
THIOGLYCOLLATE MEDIUM, BREWER |
|||
| T20-118-2kg | Alphabiosciences | 2kg | EUR 336 |
THIOGLYCOLLATE MEDIUM, BREWER |
|||
| T20-118-500g | Alphabiosciences | 500 g | EUR 128 |
 2XYT MICROBIAL MEDIUM |
|||
| X24-102-10kg | Alphabiosciences | 10 kg | EUR 614 |
2XYT MICROBIAL MEDIUM |
|||
| X24-102-2Kg | Alphabiosciences | 2 Kg | EUR 173 |
2XYT MICROBIAL MEDIUM |
|||
| X24-102-500g | Alphabiosciences | 500 g | EUR 83 |
 H Medium Broth |
|||
| DJ1019 | Bio Basic | 100g | EUR 60.88 |
|
|||
 Casein, FITC-conjugated |
|||
| 13440 | AAT Bioquest | 5 mg | EUR 219 |
|
|||
 Casein, TAMRA-conjugated |
|||
| 13441 | AAT Bioquest | 5 mg | EUR 219 |
|
|||
) Casein Antibody (Biotin) |
|||
| 20-abx106112 | Abbexa |
|
|
|
|||
) Casein Antibody (FITC) |
|||
| 20-abx107526 | Abbexa |
|
|
|
|||
) Casein Antibody (HRP) |
|||
| 20-abx108945 | Abbexa |
|
|
|
|||
Casein Antibody (HRP) |
|||
| 20-abx134736 | Abbexa |
|
|
|
|||
Casein Antibody (FITC) |
|||
| 20-abx134739 | Abbexa |
|
|
|
|||
Casein Antibody (Biotin) |
|||
| 20-abx134742 | Abbexa |
|
|
|
|||
 Casein acids hydrolysate |
|||
| abx082407-100g | Abbexa | 100 g | EUR 230 |
|
|||
 Casein enzymatic hydrolysate |
|||
| abx082408-100g | Abbexa | 100 g | EUR 217 |
|
|||
 Casein hydrolysate acid |
|||
| abx082572-500g | Abbexa | 500 g | EUR 384 |
|
|||
 CASEIN ACID HYDROLYSATE |
|||
| C03-103-10kg | Alphabiosciences | 10 kg | EUR 1708 |
CASEIN ACID HYDROLYSATE |
|||
| C03-103-2Kg | Alphabiosciences | 2 Kg | EUR 410 |
CASEIN ACID HYDROLYSATE |
|||
| C03-103-500g | Alphabiosciences | 500 g | EUR 148 |
 CASEIN ENRICHED PEPTONE |
|||
| C03-105-10kg | Alphabiosciences | 10 kg | EUR 1094 |
CASEIN ENRICHED PEPTONE |
|||
| C03-105-2Kg | Alphabiosciences | 2 Kg | EUR 277 |
CASEIN ENRICHED PEPTONE |
|||
| C03-105-500g | Alphabiosciences | 500 g | EUR 112 |
 CASEIN MEAT MIXTURE |
|||
| C03-106-10kg | Alphabiosciences | 10 kg | EUR 1018 |
CASEIN MEAT MIXTURE |
|||
| C03-106-2kg | Alphabiosciences | 2kg | EUR 260 |
CASEIN MEAT MIXTURE |
|||
| C03-106-500g | Alphabiosciences | 500 g | EUR 107 |
 Casein beta Protein |
|||
| 20-abx262498 | Abbexa |
|
|
|
|||
 Casein Polyclonal Antibody |
|||
| A57806 | EpiGentek | 100 µg | EUR 570.55 |
|
Description: fast delivery possible |
|||
 Casein sodium salt |
|||
| GE5861-100G | Glentham Life Sciences | 100 g | EUR 43 |
Casein sodium salt |
|||
| GE5861-1KG | Glentham Life Sciences | 1 kg | EUR 70 |
Casein sodium salt |
|||
| GE5861-250G | Glentham Life Sciences | 250 g | EUR 47 |
Casein sodium salt |
|||
| GE5861-500G | Glentham Life Sciences | 500 g | EUR 54 |
Steroid ligands, the forgotten triggers of nuclear receptor motion; implications for acquired resistance to endocrine remedy
Function: There’s sturdy epidemiological proof which signifies that estrogens is probably not the only steroid drivers of breast most cancers. We hypothesize that plentiful adrenal androgenic steroid precursors, appearing through the androgen receptor (AR), promote an endocrine resistant breast most cancers phenotype.
Experimental design: AR was evaluated in a main breast most cancers tissue-microarray (n=844). Androstenedione (4AD) ranges had been evaluated in serum samples (n=42) from hormone receptor constructive, post-menopausal breast most cancers.
Ranges of androgens, progesterone and estradiol had been quantified utilizing LC-MS/MS in serum from age and grade-matched recurrent and non-recurrent sufferers (n=6) pre- and post-aromatase inhibitor (AI) remedy (>12 months). Androgen and estrogen receptor signaling pathways actions had been analyzed in two impartial AI handled cohorts.
Outcomes: AR protein expression was related to favorable progression-free survival within the complete inhabitants (Wilcoxon, p<0.001). Breast most cancers sufferers pre-therapy confirmed lowering ranges of 4AD with age solely within the non-recurrent group (p<0.05).
LC-MS/MS evaluation of an AI delicate and resistant cohort demonstrated the flexibility to detect altered ranges of steroids in serum of sufferers pre and put up AI-therapy. Transcriptional evaluation confirmed an elevated ratio of AR: ER signaling pathway actions in sufferers failing AI remedy (t-test p<0.05), moreover, 4AD mediated gene adjustments related to acquired AI resistance.
Conclusions: This examine highlights the significance of inspecting the therapeutic penalties of the steroid microenvironment and demonstrable receptor activation utilizing indicative gene expression signatures.
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